使用期限*版
许可形式单机版
原产地美国
介质下载
适用平台Windows,Linux
科学软件网专注提供科研软件。截止目前,共代理千余款,软件涵盖各个学科。除了软件,科学软件网还提供课程,包含34款软件,66门课程。热门软件有:spss,stata,gams,sas,minitab,matlab,mathematica,lingo,hydrus,gms,pscad,mplus,tableau,eviews,nvivo,gtap,sequncher,simca等等。
"I am VERY impressed with Beacon Designer. I have tested the program to my satisfaction. The program saves a lot of time going to other web sites to evaluate secondary structure of primers, etc. Thanks for a great product!" -Mary L. Taub, State University of New York.
![PRIMERPLEX中文版使用教程](//l.b2b168.com/2021/09/27/16/202109271657442452204.jpg)
A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A
Yajun Song, Philippe Roumagnac, François-Xavier Weill, John Wain, Christiane DolecekCamila J. Mazzoni, Kathryn E. Holt, Mark Achtman.
Journal of Antimicrobial Chemotherapy. 2010; Aug;65(8):1631-41. doi: 10.1093/jac/dkq175.
![PRIMERPLEX中文版使用教程](//l.b2b168.com/2021/09/27/17/202109271702134388824.jpg)
"Premier Biosoft can make good software. For me Primer Premier is the best oligo design program." -Dr. Rimantas Kodzius, Genome Science Laboratory, The Institute of Physical and Chemical Research (RIKEN) Itama, Japan.
![PRIMERPLEX中文版使用教程](//l.b2b168.com/2021/09/27/17/202109271702459124734.jpg)
功能
多重SNP检测和高通量基因型
使用PrimerPlex,您可以设计SNP在旁侧的多重PCR引物、SNP在3’末端的ASPE引物以及SNPs在中心的捕获探针。PrimerPlex使您能够通过它们中每一个设计oligo,来检测一个中等大小的基因组序列的所有突变。检查这些引物和探针以进行复用,并显示佳可能的。
多重PCR引物设计
设计用于多重分析的引物集可能是一项具有挑战性的任务。PrimerPlex使用创新的和专有的算法来设计优的多重引物集,大限度地减少Tm失配所有的进行交叉反应分析,以确保特定和有效的扩增。您可以从一系列交替呈现的各种排序顺序中选择适合您的实验需求的。使用PrimerPlex,您可以灵活地设计引物,无论是朝向3'端,还是5'端,在这些端点的距离或序列内的任何地方。
对于SNP的基因型分析,该程序设计了引物旁侧突变,如SNPs、DIPs(/插入多态性)和MNPs(多核苷酸多态性)。
全面的分析支持
使用PrimerPlex为多重PCR、ASPE和直接杂交分析设计oligos。oligos被筛选为交叉反应性,以确保高信号强度,大限度地减少Tm失配。oligos筛选二聚体、运行和重复。
For SNP genotyping studies, PrimerPlex designs SNP flanking primers. The result includes a primer pair for each DNA template with product size, annealing temperature and other physio-chemical oligo properties. A graphical display of secondary structures is also available to help ascertain if they would impede the reaction.
The multiplex PCR assays are used for gene expression analysis (end point detection using gel electrophoresis), for high throughput SNP applications such as genotyping, for target enrichment required in Next Generation Sequencing, pathogen detection, strain typing, and haplotyping. PrimerPlex additionally designs optimal allele specific capture probes and Allele Specific Primer Extension (ASPE) primers for high throughput SNP genotyping assays.
科学软件网为全国大多数高校提供过产品或服务,销售和售后团队,确保您售后**!
http://turntech8843.b2b168.com