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Modern Techniques for Cross Species Detection
Real time PCR and microarrays offer a rapid, sensitive, specific and quantitative tool for the diagnosis of pathogens. Diseases are not readily distinguished based on symptoms when there is more than one pathogen in the sample. Microscopic identification requires culturing on specialized media for days to weeks. Recently, real time PCR assays were successfully used in the detection of fungal pathogens such as Rhizoctonia, Pythium, and Fusarium.
Designing a cross species assay involves the use of a multiple sequence alignment tool to identify conserved stretches of DNA and the design of a hybridization probe for detecting it. Recently there have been advances in micro-organism identification at the taxonomical level using molecular level identification techniques such as real time PCR and microarrays.
Minimal Set with Unique Primers: Choosing the Minimal set option, AlleleID® designs the smallest number of probes required to identify the sequences. The Minimal set will generally contain fewer probes than the number of sequences to be identified, and a unique combination of probes identifies each sequence. The aim of such an assay is to minimize the synthesis and overall assay costs.
Species Identification: Minimal Set with Unique Primers
设计Luminex xMAP试验
基于Luminex’s xMAP技术，AlleleID可以设计应用于悬浮阵列系统的应变-微分多重分析设计。如果您研究的是密切相关的生物体，这个功能可以帮助您在一个反应体系中进行等位基因特引物延伸（ASPE）和DHA检测。
AlleleID® Provides a Number of Options for Species Identification
Unique Probes with Unique Primers: When the probe type chosen is Unique, AlleleID® designs probes to identify each sequence in the Bind To box, one probe per sequence. The program searches for base positions that are unique to each individual sequence in the alignment. The probes are centered as close as possible over these unique bases, so that even when the mismatch is only one base, the assay succeeds.
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