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ChromasPro is suitable for DNA sequence assembly projects up to a few megabases, and basic sequence editing and analysis. It is able to assemble data from Sanger sequencers such as ABI, and 454 and Illumina next-generation sequencers, with up to 1,000,000 sequences if 8 Gb RAM is available. ChromasPro has the following features
![ChromasPro如何用](//l.b2b168.com/2019/07/19/15/20190719151119267864.jpg)
Open sequences in Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF and plain text formats.
Save sequences in .ab1, .scf, .fasta and .fastq formats.
Assemble overlapping sequences using a graphical interface, generate a consensus and highlight ambiguities for editing.
Use quality data to automatically remove low quality sequence for improved sequence assembly.
Use a reference sequence as a scaffold for assembly.
Generate restriction site and fragment maps, and list cutters, non-cutters and fragments.
Map open reading frames, aided by a G+C frame plot, and translate ORFs with one click.
Print chromatograms, restriction site and fragment maps, and open reading frame maps.
Perform nucleotide and protein BLAST searches through the NCBI web site.
Perform multiple alignments by interfacing with ClustalW.
Reverse & complement sequences and chromatograms.
Search for sequences by exact matching or optimal alignment.
Display translations when editing nucleotide sequences.
Perform reverse translations and plot nucleotide degeneracy.
Plot hydrophilicity and antigenicity of proteins.
Copy an image of a chromatogram section for pasting into documents or presentations.
![ChromasPro如何用](//l.b2b168.com/2019/07/19/15/201907191511177039654.jpg)
Fixed aligned chromatogram base numbering bug which sometimes ignored gaps in the number calculation.
Vectors used in trimming project sequences are now saved in the project file.
Clipboard copy now works on aligned sequences.
Added FASTQ format copy option.
Added trim low quality option on opening of sequence files.
Added ‘Open Project’ menu item with it’s own most recent folder.
Graphics (restriction maps, graphs, etc.) can now be saved.
Files containing multiple sequences now open in a project by default.
The restriction map options box can be recalled if closed with a map still open.
Ctrl+mou*heel now zooms the chromatogram Y-axis.
![ChromasPro如何用](//l.b2b168.com/2019/07/18/10/201907181030407449034.jpg)
1、Applied Biosystems .ab1,Staden Chromatogram(SCF和ZTR),454 SFF,FASTA,FASTQ,EMBL,GenBank,SwissProt,GenPept,GCG RSF和纯文本格式中的笔序列。
2、以.ab1,.scf,.fasta和.fastq格式保存序列。
3、使用图形界面组合重叠序列,生成共识并**显示编辑的歧义。
4、使用质量数据自动低质量序列以改进序列组装。
5、使用参考序列作为装配的支架。
6、生成限制性站点和片段映射,并列出切割器,非切割器和片段。
7、在G + C框架图的帮助下绘制开放阅读框,并一键翻译ORF。
8、打印色谱图,限制性位点和片段图,以及打开阅读框架图。
9、通过NCBI进行核苷酸和蛋白质BLAST搜索。
10、通过与ClustalW连接来执行多重对齐。
11、反向和补体序列和色谱图。
12、通过匹配或佳比对搜索序列。
13、编辑核苷酸序列时显示翻译。
14、执行反向翻译并绘制核苷酸简并。
15、绘制蛋白质的亲水性和抗原性。
16、复制色谱图部分的图像以粘贴到文档或演示文稿中。
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