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AlleleID® designs exon probes. Such probes are important to confirm the presence of an exon in the variant.
Technical resource on Gene Splicing.
How AlleleID® Designs a Cross Species/Taxa Specific Assay
AlleleID® designs both real time PCR assays, including molecular beacons, TaqMan® probes, TaqMan® MGB, and SYBR® Green primers, and microarrays for designing a universal probe for taxa discrimination.
When designing a taxa specific assay, AlleleID® designs a single common probe to identify a taxa or a group of organisms from the mix. For challenging problems when such designs are not feasible, AlleleID® attempts the design by minimizing mismatches, avoiding them especially towards the more problematic 3' end.
Molecular based techniques use species-specific probes, designed for specific regions of the target gene, for the detection and identification of bacteria. Microarrays can rapidly detect and identify multiple pathogens simultaneously in complex backgrounds.
Designing a cross species assay involves the use of a multiple sequence alignment tool to identify conserved stretches of DNA and the design of a hybridization probe for detecting it. Recently there have been advances in micro-organism identification at the taxonomical level using molecular level identification techniques such as real time PCR and microarrays.
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