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具体而有效的设计:Beacon Designer如何使其成为可能
您可以在程序中使用BLAST搜索序列并搜索模板结构。在设计引物和探针时使用这两种搜索的结果。在设计过程中,会自动避免出现明显的交叉同源性和模板结构的区域。
![Beacon designer正版软件怎么用](//l.b2b168.com/2021/09/27/17/202109271731574995474.jpg)
Design TaqMan® Probes
The TaqMan® assay design module in Beacon Designer™ can design TaqMan® probes suitable for single sequence as well as multiplex experiments. Beacon Designer™ supports multiplexing for up to four sequences, with the ability to lock an assay for a reference gene. The primers and probes designed for other sequences in the multiplex set will take into account the Tm and cross dimer values for this assay as a reference.
![Beacon designer正版软件怎么用](//l.b2b168.com/2021/09/27/17/202109271731576782884.jpg)
MethyLight Assay Design for DNA Methylation Profiling
MethyLight assay, a TaqMan® probes based methylation profiling technique, can be effectively used for DNA methylation profiling, measure DNA methylation or detect methylated alleles in presence of the unmethylated ones. Due to the specificity of TaqMan® based assays, it is much more accurate than the end point detection assay such as methyl specific PCR (MSP).
![Beacon designer正版软件怎么用](//l.b2b168.com/2021/09/27/17/202109271731574380764.jpg)
HRMA试验设计
Beacon Designer为突变检测提供了一个综合解决方案。高分辨率熔解分析(HRMA)是一种比基于**腺的基因型分析更具有成本效益的方法。该程序使用专有算法,可以设计出检测突变的佳引物。
高分辨率的熔解分析引物被设计成侧翼感兴趣突变,产生具有可检测的熔融温度变化的短可能的扩增子。引物设计避免了模板二级结构,保证有效引物延伸。设计的HRMA引物可以在NCBI的核苷酸数据库中进行搜索,以检验其特。还可以对预先设计或发布的引物进行分析。
Design specific and efficient oligos for all major qPCR assays
Design successful SYBR® Green, TaqMan®, HRMA Primers, MethyLight TaqMan®, LNA™ spiked TaqMan®, molecular beacons for both standard and NASBA® assays, Scorpions® and FRET assays by automatically interpreting BLAST results and avoiding template structures.
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