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When it is not possible to identify a significant conserved region for a set of sequences, AlleleID® attempts to design a "Mismatch Tolerant" probe. The probe may include up to the specified number of mismatched bases which are located as close as possible to the 5' end.
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Modern Techniques for Cross Species Detection
Real time PCR and microarrays offer a rapid, sensitive, specific and quantitative tool for the diagnosis of pathogens. Diseases are not readily distinguished based on symptoms when there is more than one pathogen in the sample. Microscopic identification requires culturing on specialized media for days to weeks. Recently, real time PCR assays were successfully used in the detection of fungal pathogens such as Rhizoctonia, Pythium, and Fusarium.
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Specifying Sequences: Specify the sequences you would like to detect in the mix by moving them to the "Bind To" box.
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