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"Our success rate has been over 90% when we used the SYBR® Green primers and TaqMan® probes designed with Beacon Designer." -Dr. Michael Sheldon, Baylor College of Medicine, Houston, Texas.
A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A
Yajun Song, Philippe Roumagnac, François-Xavier Weill, John Wain, Christiane DolecekCamila J. Mazzoni, Kathryn E. Holt, Mark Achtman.
Journal of Antimicrobial Chemotherapy. 2010; Aug;65(8):1631-41. doi: 10.1093/jac/dkq175.
For SNP genotyping assays, the program designs primers flanking mutations such as SNPs, DIPs (Deletion/Insertion Polymorphisms) and MNPs (Multiple Nucleotide Polymorphisms).
"I used Beacon Designer to design primers for several targets. All the designed primers had an efficiency of over 90% and they worked well for me. Excellent R^2 and melt curves were obtained for these primers." -Mr. Islam El-Awaad, Institut für Pharmazeutische Biologie, Technische Universität Braunschweig, Germany.
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