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For SNP genotyping assays, the program designs primers flanking mutations such as SNPs, DIPs (Deletion/Insertion Polymorphisms) and MNPs (Multiple Nucleotide Polymorphisms).
多重PCR检测用于基因表达分析（使用凝胶电泳的终点检测），用于高通量SNP应用，如基因分型，用于下一代测序所需的目标浓缩、病原体检测、菌株分型和单倍型分型。PrimerPlex还设计了优等位基因特捕捉探针和Allele Specific Primer Extension (ASPE)引物用于高通量SNP基因型分析。
PrimerPlex 多重PCR和高通量SNP基因分型和分析的Oligo设计
对于SNP的基因型研究，PrimerPlex设计了SNP的旁侧引物。其结果包括每个DNA模板的引物对，包括产品尺寸、退火温度等物理化学寡聚物性能。此外，二级结构的图形显示也可用以帮助确定它们是否会阻碍反应。
多重PCR检测用于基因表达分析（使用凝胶电泳的终点检测），用于高通量SNP应用，如基因分型，用于下一代测序所需的目标浓缩、病原体检测、菌株分型和单倍型分型。PrimerPlex还设计了优等位基因特捕捉探针和Allele Specific Primer Extension (ASPE)引物用于高通量SNP基因型分析。
Primer Specificity
Evaluate Pre-Designed Primers and Probes
Addition of MagPlex TAGs
"We love your program - it has saved time in that we don't have to use 3 or 4 different programs to determine how well the probes we have designed will bind to other sequences. And it has saved money because I don't have to purchase several expensive labeled probes to test which one will work the best. With Beacon Designer™ 2, we can design specific probes efficiently and with the multiplex function save even more time and feel confident about the results. Certainly, Beacon Designer™ 2 will be able to design effective probes for detection of single base pair differences." -Dr. Gale Newman, Dept. Micro, Biochem, Immuno Morehouse School of Medicine, Atlanta, GA.
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