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Versatile Primer Design & Analysis
Using the manual primer search option, a user can
click any nucleotide on the template sequence to get
primers at the location of their choice. Primer Premier
instantly analyzes the primer properties. The program
also evaluates the properties of pre-designed primers
and ranks them for you to adjudge how far the primers
are from their desired target values. This feature is
especially useful if you are working with published or
pre-designed well proven primers. For any given
primer, it even designs a compatible forward or
reverse primer.
![PRIMER PREMIER正版软件代理商](//l.b2b168.com/2019/07/18/10/201907181030442496294.jpg)
Local & Desktop BLAST Options
You can BLAST your sequences against local custom databases using the Local and Desktop BLAST options. Create a database of sequences in text or FASTA format on your computer and BLAST your query sequence from Primer Premier. The results are then used for primer design. This support is especially useful if you do not want to submit your data on the Internet or if you are working with unpublished information.
![PRIMER PREMIER正版软件代理商](//l.b2b168.com/2021/09/27/17/20210927170546309054.jpg)
Primer Premier PCR引物设计软件
标准PCR分析的引物设计
Primer Premier是设计和分析PCR引物的全面的软件。Primer Premier通过利用接近的热力学算法和的熔化温度,找到优的PCR、多重和SNP基因分型引物。引物被筛选的二级结构、二聚体、发夹、同源性和物理性质,然后报告佳的序列,按顺序排列。配备了一个方便的计算器,您可以很*地操作序列并分析您的引物设计的结果。从NCBI加载感兴趣的基因,选择一个搜索范围,让Primer Premier为您挑选好的引物。
Primer Design for SNP Genotyping Assays
With Primer Premier, you can load sequences from dbSNP and have the primers designed flanking the SNP selected. Hundreds of unpublished SNPs can also be loaded by specifying them as variation features in standard GenBank/dbSNP files. After specifying the SNPs, primers can be designed to amplify them for detection using a probe-based chemistry.
![PRIMER PREMIER正版软件代理商](//l.b2b168.com/2021/09/27/17/202109271704167399824.jpg)
Calculator
A built in calculator calculates the GC%, displays the base count and enables you to obtain the complementary and the reverse complementary strands.
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