使用期限*
许可形式单机
原产地美国
介质下载
适用平台windows
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Choices for Primer Design: There are two choices available for primer design, Unique and Optimal. When you choose Unique, the program designs a primer pair to amplify each sequence. For Optimal, the program minimizes the number of primers required to amplify all sequences. The goal is to minimize the synthesis and overall assay costs.
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When it is not possible to identify a significant conserved region for a set of sequences, AlleleID® attempts to design a "Mismatch Tolerant" probe. The probe may include up to the specified number of mismatched bases which are located as close as possible to the 5' end.
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Although these double-stranded DNA-binding dyes provide the simplest and cheapest option for real time PCR, the principal drawback to intercalation based detection of PCR product accumulation is that both specific and nonspecific products generate **.
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物种分析/跨物种实验/等位基因分析
AlleleID使用ClustalW对序列进行排列,并分析保存和物种特定区域。您可以使用程序进行实时PCR引物设计(包含SYBR Green引物设计)和双标签探针设计(TaqMan probes,
TaqMan MGB and molecular beacons)。这些检测方法只用于进行菌株检测或从混合菌株中找到感兴趣的物种。为了进行交叉物种试验,AlleleID通过识别保守区域来设计通用探针。对于相关的生物体,当研究物种的基因组草图不可用时,可以使用AlleleID来研究其基因表达。这些强大的功能可以帮助解决了很多具有挑战性的难题,例如的检测、、定量以及污染物、环境等的监测。
设计Luminex xMAP试验
基于Luminex’s xMAP技术,AlleleID可以设计应用于悬浮阵列系统的应变-微分多重分析设计。如果您研究的是密切相关的生物体,这个功能可以帮助您在一个反应体系中进行等位基因特引物延伸(ASPE)和DHA检测。
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