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The [1] displayed in the first pane indicates that only a single peptide has been highlighted. You can highlight multiple sequences by holding down the shift button while selecting additional regions. Up to three regions can be selected at a given time. The region covered will only be shown for the last selection, while the mass will be for the total..
肽窗口列出了从给定蛋白质产生的所有肽以及大量(>20)物理化学参数。
充电-单次/多次/负/正
肽数
位置
液相色谱指数
理论PI
布尔和布雷斯指数
序列-1/3字母等
肽可以通过部分解理生成(图中蓝色上标表示),改性界点、改性残渣,即使是部分修改也得到有限的支持。为便于在序列窗口中引用着色的特定残留物而被带到这个窗口。用户可以配置显示的实际参数。通过单击标题,可以对任何列进行排序。*二次单击将反转排序。通过工具栏和/或弹出菜单(鼠标右键单击),您可以访问与消化(如模拟的HPLC反相色谱图)或当前所选肽(MS/MS裂解、肽信息、电荷与ph图)相关的其他功能。
以及精美的图片都可以轻松完成。
A12 The white on blue ‘i’ opens the sequence information window, which gives you file and statistical information, calculated indices (pI, absorption etc.), amino acid composition and multiply charged masses. The drop-down arrow open a menu giving direct access to the various pages in the information window.
A5 The leftmost panel shows the total mass of the protein in Daltons. The button next to it shows whether it is the average mass (Av. – blue) or monoisotopic mass (Mo. – red). You can change the mass type by clicking on the Av./Mo. button.
The next two panels from the left can show either of two states:
A6 If no peptide is selected the mass of the amino acid under the cursor (i.e. the residue mass + 18 Da) will be displayed in the left-hand panel and the right-hand panel will show the residue number (if the protein is a multi-chain protein, the first chain will be labeled ‘a’, the second ‘b’ etc (i.e. 80b is residue number 80 in the second chain counting from the N-terminus).
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