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Multiplex PCR Primer Design
Designing primer sets that work well for a multiplex assay can be a challenging task. PrimerPlex uses innovative and proprietary algorithms to design optimal multiplex primer sets, minimizing Tm mismatches. All sets are analyzed for cross reactivity to ensure specific and efficient amplification. You can choose the set best suited for your experimental needs from a list of alternates presented in variously sortable order. Using PrimerPlex, you have the flexibility of designing primers either towards the 3' end, the 5' end, at a specified distance from these ends or anywhere within the sequence.
![PRIMERPLEX正版软件实用教程](//l.b2b168.com/2021/09/27/17/202109271702571956954.jpg)
Avoiding homologous regions makes the oligos highly specific and avoiding template structures improves the efficiency of the designed primers.
![PRIMERPLEX正版软件实用教程](//l.b2b168.com/2021/09/27/17/20210927170213393924.jpg)
"The SYBR Green primers designed using Beacon Designer worked extremely well. Pretty good amplification factor of over 90% was observed with no primer-dimers." -Mr. Adelumola Oladeinde, University of Georgia, United States.
![PRIMERPLEX正版软件实用教程](//l.b2b168.com/2021/09/27/17/202109271702458721864.jpg)
Manual Primer Search
Primer Premier offers complete control over primer design to the user. Using the manual primer search option, a user can select any position on the template sequence to design a sense or an anti-sense primer. Primer Premier instantly calculates and displays the properties of the new primers. The primers finally selected by a user are then shown in the Sequence View on the Main window.
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