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A multiple sequence-alignment or (MSA) is not the same as sequence assembly so don't be surprised if your contigs and multiple-sequence alignments look different.
New and enhanced features for all of your DNA sequence analysis.
Sanger:
Send primer pair sequences from Primer-BLAST runs in Sequencher Connections to your Sequencher project.
You can do BLAST searches in a Connections Session for individual sequences. Channels can be set up to search the NCBI databases with different parameters or databases used on each channel. Local-BLAST channels can be set up to search databases on your machine. You can also set up a Primer-BLAST channel to design primers over a region of interest and send those primer sequences to your Sequencher product. The primer sequences will have colored bases and the primer feature applied to them. The Schematic shows the results from multiple channels in a single window to allow you to compare the results from different runs.
With more than a decade of commercial availability behind them, microarrays are still a popular method of gene expression profiling despite the increasing popularity of RNA-Seq.
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