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Avoiding homologous regions makes the oligos highly specific and avoiding template structures improves the efficiency of the designed primers.
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Molecular dysexpression in gastric cancer revealed by integrated analysis of transcriptome data
Xiaomei Li, Weiwei Dong, Xueling Qu, Huixia Zhao, Shuo Wang, Yixin Hao, Qiuwen Li, Jianhua Zhu, Min Ye, Wenhua Xiao.
Oncology Letters. 2017; May;13(5):3177-3185. doi: 10.3892/ol.2017.5798.
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