使用期限*
许可形式单机
原产地美国
介质下载
适用平台windows
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功能特征
物种试验
基因芯片的基因拼接/选择性剪接
外显子连接引物设计
实时PCR引物设计
TaqMan探针设计
SyBR绿色底漆设计
分子信标设计
FRET探针设计
微阵列探针设计
精密化验设计
数据库支持
输入输出格式
Real Time PCR Primer and Probe Design with AlleleID® & Beacon Designer™
Minimal Probes with Optimal Primers
For Optimal, the program minimizes the number of primers required to amplify all sequences. The goal is to minimize the synthesis and overall assay costs.
Taxa Specific/Cross Species identification: Probes with Mismatched Bases and Optimal Primers
Minimal Probes with Optimal Primers: When the homology among the aligned sequences is very low and both the designs mentioned above (a single taxa specific probe set or probe set with mismatch tolerance) are not feasible, AlleleID® designs the minimum number of probes possible to detect all the sequences in an alignment. The program designs probes for the majority consensus first and then for the minority consensus and remaining individual sequences. This design is very useful, especially for studying phylogenetic relationships among sequences obtained from different geographical locations.
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