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Manual Primer Search
Primer Premier offers complete control over primer design to the user. Using the manual primer search option, a user can select any position on the template sequence to design a sense or an anti-sense primer. Primer Premier instantly calculates and displays the properties of the new primers. The primers finally selected by a user are then shown in the Sequence View on the Main window.
Razieh Fatehi, Sharifeh Khosravi, Maryam Abedi, Rasoul Salehi, Yousof Gheisari.
Journal of Research in Medical Sciences. 2017; Sep 26;22:102. doi: 10.4103/jrms.JRMS_136_17.
Heterozygosity analysis of polycystic kidney disease 1 gene microsatellite markers for linkage analysis of autosomal dominant polycystic kidney disease type 1 in the iranian population
"We have designed several primer/probe sets for reverse-transcription quantitative PCR using TaqMan. All have worked very well. We particularly like the way that the primers/probes are 'scored' as best, good or poor, it is much more convenient than other programs we have used. We also like the integrated BLAST feature. We are very satisfied customers and have recommended the product to our colleagues and collaborators." -Ms. Laura O'Hara, University of Cambridge, UK.
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