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"I really liked AlleleID. I found it very useful and intuitive. I designed several sets of SYBR® Green primers using the software. I tested them and they all worked very well." -Dr. Jeannette Loram, Bermuda Institute of Ocean Sciences, Bermuda.
![PRIMERPLEX实用教程](//l.b2b168.com/2019/07/19/15/201907191511177039654.jpg)
For SNP genotyping assays, the program designs primers flanking mutations such as SNPs, DIPs (Deletion/Insertion Polymorphisms) and MNPs (Multiple Nucleotide Polymorphisms).
![PRIMERPLEX实用教程](//l.b2b168.com/2019/07/18/10/201907181030369734964.jpg)
Primer extension may be hindered due to the presence of template structures at extension temperature. To avoid all such regions, where the template may fold upon itself, the program utilizes a proprietary algorithm to check for possible secondary structures within the template at a folding temperature you specify. The regions involved in the formation of a secondary structure are underlined in the sequence view and are avoided while designing primers.
![PRIMERPLEX实用教程](//l.b2b168.com/2021/09/27/17/202109271702571956954.jpg)
"I truly enjoyed the program over all these years (see ref. in Fetissov et all., Reg. Peptides 119:113-124, 2004)." -Dr. Serguei Fetissov, Karolinska Institute, Sweden.
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