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‘Invert sequence’ will result in highlighting of sequences found in both N- and C-terminal directions (e.g. if entering KLGFT both the sequence KLGFT and the sequence TFGLK will be highlighted). Useful for searching for a ms/ms sequence tags (you may not know whether it is a y-ion or a b-ion series). Isobaric resides will, if checked, highlight both kinds of residues, i.e. if the ‘Q/K’ checkbox is checked and the sequence LKT is entered, the sequence LQT will also be highlighted. Again, this is for the benefit of ms/ms sequence tags.
![GPMAW学习班](//l.b2b168.com/2019/07/18/10/201907181030385050684.jpg)
Underlines.
Underlines are different from highlights and can be a different way of drawing attention to specific residues/sequences:
The can be persistent, i.e. you can save the underline information along with the sequence (File|Save w. highlights).
You only have a single color to work with (red by default Setup).
![GPMAW学习班](//l.b2b168.com/2019/07/18/10/201907181030369734964.jpg)
A15 The Coverage button only appears when the sequence has a corresponding coverage map associated. This can be generated from mass searches, see later.
![GPMAW学习班](//l.b2b168.com/2019/07/18/10/201907181030459619184.jpg)
f part of the sequence is highlighted, the left panel shows the mass of the selected peptide and the right panel shows the first and last residues of the selection (see next section).
Note that the mass value displayed is M, not M+H.
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