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You deselect all regions by clicking once in the sequence without holding down the Shift key.
生物信息学:可显示多种图形、疏水性、点图、二级结构预测。可以在本地数据库上执行BLAST搜索。使用ClustalW的多重对齐。CustalW可执行文件现在是GPMAw包的一部分,但是在以前的版本中,您必须自己安装。
序列窗口
序列窗口是GPMAW的核心窗口并默认显示一个序列。从这里,您可以调用大多数与序列相关的函数(通过菜单、工具栏或弹出菜单)。
A5 The leftmost panel shows the total mass of the protein in Daltons. The button next to it shows whether it is the average mass (Av. – blue) or monoisotopic mass (Mo. – red). You can change the mass type by clicking on the Av./Mo. button.
The next two panels from the left can show either of two states:
A6 If no peptide is selected the mass of the amino acid under the cursor (i.e. the residue mass + 18 Da) will be displayed in the left-hand panel and the right-hand panel will show the residue number (if the protein is a multi-chain protein, the first chain will be labeled ‘a’, the second ‘b’ etc (i.e. 80b is residue number 80 in the second chain counting from the N-terminus).
Fragment button. Create (ms/ms) fragments of your protein. If part of the main sequence is highlighted, this part will be taken as the fragment peptide. If no selection has been made, the whole sequence is taken as input. If the sequence is longer than 50 residues you will be asked for confirmation before the first 400 residues are used as input.
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