使用期限*
许可形式单机
原产地美国
介质下载
适用平台windows
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AlleleID为PamGene检测设计了mRNA特,DNA特和突变特MLPA探针。这将使PamChip用户在使用PamGene高精度检测平台时设计自己感兴趣的基因探针检测。结合这两种技术,拷贝数的变化和突变可以很*地在6小时内检测到。
功能特征
物种试验
基因芯片的基因拼接/选择性剪接
外显子连接引物设计
实时PCR引物设计
TaqMan探针设计
SyBR绿色底漆设计
分子信标设计
FRET探针设计
微阵列探针设计
精密化验设计
数据库支持
输入输出格式
Platforms supported for Windows: XP/Vista/Windows 7/Windows 8/Windows 10
Tested on Mac OS 10.6/10.7/10.8/10.9
![AlleleID学习班](//l.b2b168.com/2019/07/18/10/201907181030442496294.jpg)
Specifying Sequences: Specify the sequences you would like to detect in the mix by moving them to the "Bind To" box.
![AlleleID学习班](//l.b2b168.com/2019/07/18/10/201907181030484134514.jpg)
Taxa Specific/Cross Species Identification: Conserved Probe with Optimal Primers
Conserved Probe with Optimal Primers: AlleleID® designs a primer pair that would amplify the maximum number of sequences possible in an alignment. There are two choices available for designing primers, Unique and Optimal. For "Unique", the program designs a primer pair to amplify each sequence. Each primer is completely homologous to its template. For "Optimal', the program minimizes the number of primers required to amplify all sequences; taking advantage of the fact that primers can bind to a sequence and amplify it even if they contain a limited number of mismatched bases especially towards the 5' end of the primer.
![AlleleID学习班](//l.b2b168.com/2019/07/18/10/201907181030476922774.jpg)
Real Time PCR & Microarray Primer/Probe Design for Cross Species and Taxa Specific Applications
For cross species assays, AlleleID® identifies the conserved regions to design a universal probe. This functionality is especially useful to detect a species or a taxa when designing a diagnostic assay. This functionality is also useful to help study gene expression when genome draft of the organism under study is not available.
When it is not possible to identify a significant conserved region for a set of sequences, AlleleID® attempts to design a "Mismatch Tolerant" probe. The probe may include up to the specified number of mismatched bases which are located as close as possible to the 5' end.
Also included is the "Minimal Set" option that helps you design minimum number of probe sets that uniquely identify a sequence reducing the overall assay cost.
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