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MethyLight Assay Design for DNA Methylation Profiling
MethyLight assay, a TaqMan® probes based methylation profiling technique, can be effectively used for DNA methylation profiling, measure DNA methylation or detect methylated alleles in presence of the unmethylated ones. Due to the specificity of TaqMan® based assays, it is much more accurate than the end point detection assay such as methyl specific PCR (MSP).
Beacon Designer™ outputs many TaqMan® probe-primer sets for the user to choose from.
DNA Methylation Mechanism
In DNA, methylation usually occurs in the CpG islands, a CG rich region, upstream of the promoter region. The letter "p" here signifies that the C and G are connected by a phosphodiester bond. In humans, DNA methylation is carried out by a group of enzymes called DNA methyltransferases. These enzymes not only determine the DNA methylation patterns during the early development, but are also responsible for copying these patterns to the strands generated from DNA replication.
具体而有效的设计：Beacon Designer如何使其成为可能
您可以在程序中使用BLAST搜索序列并搜索模板结构。在设计引物和探针时使用这两种搜索的结果。在设计过程中，会自动避免出现明显的交叉同源性和模板结构的区域。
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