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Sequence handling: Import of sequences from a number of different formats with direct database search in Entrez and in local databases (FastA format and Swiss-Prot). Sequences can be saved in local files (databases) for future reference.
From the sequence window a large number of actions can be performed. Sequences can be exported in FastA format (either singly or all sequences at once) for easy transfer to other programs.
Mass analysis: The protein can be cleaved by automatic methods (e.g. a flexible nomenclature for defining enzyme actions) or manually. The peptides are displayed with a number of parameters (various mass values - mono, ave, charges - Bull&Breese index, HPLC index, pI, charge) and can be further worked upon (e.g. cross-linked, new cleavage).
Peptide mass searches can be performed on any local database in FastA format.
Bioinformatics: A number of graphs can be displayed, hydrophobicity, dot-plot, secondary structure prediction. BLAST searches can be performed on local databases. Multiple alignment using ClustalW.
The CustalW executable is now part of the GPMAw package, but on previous versions you have to install yourself. Read here how.
Molar Ext./Abs.@280: Theoretical extinction coefficient / absorption of the protein at 280 nm.
Highlights: Percentage of the sequence, which is inverted (highlighted) or underlined, updated dynamically.
Sel. mass: Mass of the selected (highlighted) part of the sequence. Shown as singly, doubly and triply charged peptide (i.e. residue mass + 18 + charge). Updated dynamically. Note that unlike the peptide mass shown in the toolbar of the sequence window, this is the charged ion. More on peptide selections in part 3.
ClustalW in GPMAW has not been strongly advocated because the version of ClustalW that could be downloaded was not compatible with Windows XP. However, this has been corrected, and the installation description follows.
The GPMAW program is primarily intended as a tool for mass spectrometric analysis of proteins and peptides. However, a number of other bioinformatics tools have been included, so the use of the program extends far beyond simple mass analysis.
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