使用期限*
许可形式单机
原产地美国
介质下载
适用平台windows
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功能特征
物种试验
基因芯片的基因拼接/选择性剪接
外显子连接引物设计
实时PCR引物设计
TaqMan探针设计
SyBR绿色底漆设计
分子信标设计
FRET探针设计
微阵列探针设计
精密化验设计
数据库支持
输入输出格式
AlleleID是一款旨在解决、病原体检测或物种的综合性桌面工具。以CulsSTW多序列比对为核心，AlleleID可以设计用于物种识别/交叉物种的基因芯片探针以及用于实时PCR的SYBR Green, TaqMan MGB, TaqMan探针, Molecular Beacons以及实时PCR引物。AlleleID还提供了支持可变剪切片段检测的微阵列实验设计。
检测成功的复杂算法
高度特寡核苷酸通过回避自动注释BLAST搜索结果找到的显著同源性区域而设计。实时定量PCR引物和探针阻止模板形成二级结构提升效率。"Minimal Set"，该程序具创新性的特点之一，有助于设计少数量的等位基因特寡核苷酸引物和双标记探针，其特地识别来自混合物的所需物种/菌株/类群中的每一个，降低化验成本。对于类群或跨种分析，当类群或类群高度不同时，该特征尤其有用。对于一组预先设计的、经过验证的引物，AlleleID可以设计兼容的引物和探针的其余序列的物种或类群特检测。
广泛支持实时或定量PCR检测和SNP/表达微阵列
AlleleID可以设计优的SYBR Green引物、TaqMan探针、TaqMan Minor Groove Bingding
(MGB)探针、FRET探针或者分子信标，用于实时定量PCR差异基因表达SNP基因型分类试验。您也可以设计实时PCR引物和双标记探针多达一万个序列在一个单一的运行SNP检测或表达微阵列。
设计Luminex xMAP试验
基于Luminex’s xMAP技术，AlleleID可以设计应用于悬浮阵列系统的应变-微分多重分析设计。如果您研究的是密切相关的生物体，这个功能可以帮助您在一个反应体系中进行等位基因特引物延伸（ASPE）和DHA检测。
多路连接依赖探针扩增（MLPA）检测拷贝数和突变检测
AlleleID是设计MLPA合成探针或者由MRC Holland引入的多重连接依赖探针扩增技术的程序，当合适的工具包不可用时，研究人员能够扩展这项技术。这是一种快速增长的高通量基因图谱分析方法。可以检测外显子缺失、拷贝数变化和CpG甲基化模式。具有成本效益合适、敏感性高、特好和可再生性好等特点。
AlleleID为PamGene检测设计了mRNA特,DNA特和突变特MLPA探针。这将使PamChip用户在使用PamGene高精度检测平台时设计自己感兴趣的基因探针检测。结合这两种技术，拷贝数的变化和突变可以很*地在6小时内检测到。
Beacon Designer™ is a comprehensive real time PCR primer and probe design tool for designing single template and multiplex assays. Real time PCR chemistries supported include Molecular beacons, TaqMan®, FRET, Scorpions® and SYBR® Green. It designs molecular beacons for standard and NASBA® assays and designs LNA spiked Taqman® probes as well. AlleleID® supports all the major features of Beacon Designer™ except NASBA® assay, LNA™ spiked Taqman® probe design and multiplex assay design. TaqMan® MGB probe design is unique to AlleleID® (whereas regular TaqMan® probes based assays can be designed using both AlleleID® and Beacon Designer™. AlleleID® is written for advance real time PCR applications such as oligo design for species identification and taxa discrimination. It includes a multiple sequence alignment module for identifying conserved and species specific regions. AlleleID® also includes support for real time PCR primer design over exon junctions for selective amplification of cDNA.
Limitations of End-point PCR
In a PCR reaction as the reaction progresses, the reagents are being consumed as a result of amplification. Now the PCR product is no longer being doubled at each cycle due to this reagent constraint. This depletion will occur at different rates for each replicate. Thus, the samples begin to diverge in their quantities. This diminished amplification is the linear phase of the reaction. The plateau for each tube will differ due to the different reaction kinetics for each sample. It is in this phase where traditional PCR takes its measurement, also known as the end-point. This End-Point Detection has some problems such as low resolution, poor precision, low sensitivity and the need for post PCR processing. Also, the results of this detection are not expressed in numbers and there is no scope for automation.
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