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When reading back a sequence already saved in a sequence library, there is a difference between opening a library file containing a single sequence and one containing multiple sequences. If the library file contains a single sequence, it will be read into GPMAW immediately you select the file, while you will be asked to select a sequence from a list if the library file contains multiple sequences.
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When you highlight a residue you are changing the background color. When you modify or underline residues the colors of the letters are changed (by default modified residues are colored red), so be careful when selecting colors for background as residues will ‘disappear’ if front and back colors are identical.
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The status bar is initially green, but turns yellow and red as you get close to full capacity of the file. Furthermore, when a protein is selected, the basic information is displayed in the right-hand information box.
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ClustalW in GPMAW has not been strongly advocated because the version of ClustalW that could be downloaded was not compatible with Windows XP. However, this has been corrected, and the installation description follows.
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