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"We were frustrated because of low PCR product. We bought Primer Premier and had high yield from the very first nested PCR we did using the program." -Dr. Patricia Commers, Vanderbilt University.
A Comprehensive PCR Primer Design Software
Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in a ranked order. Load the gene of interest from NCBI, select a search range, sit back and let Primer Premier pick the best possible primers for you.
多重PCR检测用于基因表达分析(使用凝胶电泳的终点检测),用于高通量SNP应用,如基因分型,用于下一代测序所需的目标浓缩、病原体检测、菌株分型和单倍型分型。PrimerPlex还设计了优等位基因特捕捉探针和Allele Specific Primer Extension (ASPE)引物用于高通量SNP基因型分析。
"I found the program was extremely user friendly and fast. I am very happy with the quality of the results, much better than any other program that I tested" -Dr. Ourania Kosti, Department of Molecular Endocrinology, Division of General and Developmental Medicine, Barts and the London, Queen Mary School of Medicine and Dentistry, London, UK.
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