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You can use pre-designed or published or well proven forward or reverse primers and Beacon Designer™ will evaluate them for you. For example, for a given forward primer, Beacon Designer™ would design the reverse primer for SYBR® Green assays (and would design both the reverse primer and the TaqMan® probe for TaqMan® assays). All these primers are rated according to a ranking algorithm which determines how closely the specified primer pair and primer search parameters match the target values.
TaqMan® Probes
Use Beacon Designer™ for optimal TaqMan® probes that help you study the differential gene expression or study SNP (single nucleotide polymorphisms) using TaqMan® SNP genotyping assays. You can also choose to design the increasingly popular Locked Nucleic Acid (LNA™) substituted TaqMan® probes, which are more stable than standard TaqMan® probes. To study DNA methylation, Beacon Designer™ helps design primers and TaqMan® probes for MethyLight assays. MethyLight is a high throughput assay used to discriminate between methylated and unmethylated DNA. Using Beacon Designer™, you can design suitable control primers and probes by designing oligos for the methylated and the unmethylated strands, and for the untreated DNA sequence. With any of these chemistries, you could evaluate a pre-designed/published primers or TaqMan® probes. The design results are reported in a ranked order, giving you a list of alternates to choose from. Depending on the oligo properties, a rating is assigned. Please read the Rating section in the online help for details.
Automate Your Real Time PCR Primer and Probe Design
Beacon Designer™ automates the design of real time primers and probes. It is used by molecular biologists worldwide to design successful real time PCR assays. It saves the time and the money involved in failed experiments. Beacon Designer™ is a flexible solution to your real time primer and probe design needs and pays for itself many times over.
可用的设计选项
SYBR Green试验设计
SYBR Green可能是实时PCR化学中常用的。Beacon Designer设计SYBR Green PCR引物，帮助您评估预先设计的SYBR Green PCR引物集。您可以完全控制设计参数，或者您可以使用默认值，在经过大量的研究和与的交流之后选择。您甚至可以为您的特定研究需求选择您自己的默认设计参数。
除了在感兴趣的基因上定位引物外，信标设计者还可以设计外显子或外显子内含子连接的引物。这些引物设计的外显子内含子连接有助于选择性帮助从gDNA选择性扩增cDNA。
2020年，北京天演融智软件有限公司申请高等教育司产学合作协同育人项目，“大数据”和“机器学习”师资培训项目，以及基于OBE的教考分离改革与教学评测项目已获得批准。我们将会跟更多的高校合作，产学融合协同育人。
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