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Sequencher makes traditional sequence assembly easy while keeping you in control.
Trim your sequences for poor quality data with ease. You can even maintain a library of trim criteria to make life even easier.
With intuitive controls you can select the best algorithm for your data including Assemble to Reference. And if you are working with multiple samples from different sources then automate the assembly using Assemble by Name.
Editing your data has never been simpler with tools to help you locate and deal with ambiguities, check for heterozygotes and move around your data.
Use confidence values to assist in trimming data, quality checks, and SNP detection to improve the quality of your results.
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Your new primers are automatically tagged with a GenBank style feature key that makes them easier to see in an alignment. The primers are color-coded by direction for easy identification any time you use them AND you will never get them confused thanks to their naming convention.
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Assemble to Reference
The Reference sequence is a powerful feature, core to many aspects of sequencing and sequence analysis. Whether you are SNP hunting, engaged in Forensics, phylogenetic studies, medical genetics or population studies you will want to use the Reference Sequence feature.
Import a sequence in GenBank format and its feature table will be applied to the sequence, and mark it as a Reference Sequence. Assembling to the Reference Sequence means you are able to compare your reads to the archetypal Reference Sequence. If you are working with multiple samples from different sources you can even use Assemble by Name to automate your work.
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Sequencher DNA序列分析软件是科学家们*的工具。持续发展和改进**过25年,Sequencher提供了**的功能特征,成千上万的出版物都有相关的应用介绍。新手用户可以以少的时间投资产生结果,而有经验的用户会惊叹于功能的深度和控制。Sequencher自带各种专有算法,可以为Sanger, Next-Gen Sequencing (NGS)和RNA-Seq序列数据生成结果。
甲基化研究
几十年来,DNA甲基化被认为在基因表达中起着重要的作用。当用亚硫酸氢盐处理DNA时,除非胞嘧啶甲基化,任何胞嘧啶都被转换成尿嘧啶。GSNAP将从亚硫酸氢盐处理的DNA测序获得的下一代测序读数校准为参考序列,以不掩盖真正错配的方式排列它们,但当用亚硫酸氢盐处理未甲基化DNA时仍然暴露发生的C到T失配。
RNA耐受性比对
GSNAP已经更新,现在包含A-G容错对齐模式。使用这种模式的情况下,您的mRNA可能已被ADAR基因编辑,使腺苷转化为肌苷。当测序时,I被视为G。该版本还引入了用于新的RNA耐受模式和甲基化分析的滞留和非滞留模式。当您的实验室协议允许5’到3’基因组读取和它们在每个链上的反向互补时,使用非搁浅模式。
查看器
Tablet是下一代序列比对的高性能查看器。以许多不同的模式查看和审查您的Maq, GSNAP,
BWA-MEN或Velvet结果,用不同的颜色和阅读方向**显示。获取个别读数和读取成对的信息,以及排列读取的堆叠以显示配对。Tablet可以控制转换、缩放以及**变异基,以便让您的阅读变得*。
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