使用期限*
许可形式单机
原产地美国
介质下载
适用平台windows
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AlleleID为PamGene检测设计了mRNA特,DNA特和突变特MLPA探针。这将使PamChip用户在使用PamGene高精度检测平台时设计自己感兴趣的基因探针检测。结合这两种技术，拷贝数的变化和突变可以很*地在6小时内检测到。
AlleleID是一款旨在解决、病原体检测或物种的综合性桌面工具。以CulsSTW多序列比对为核心，AlleleID可以设计用于物种识别/交叉物种的基因芯片探针以及用于实时PCR的SYBR Green, TaqMan MGB, TaqMan探针, Molecular Beacons以及实时PCR引物。AlleleID还提供了支持可变剪切片段检测的微阵列实验设计。
检测成功的复杂算法
高度特寡核苷酸通过回避自动注释BLAST搜索结果找到的显著同源性区域而设计。实时定量PCR引物和探针阻止模板形成二级结构提升效率。"Minimal Set"，该程序具创新性的特点之一，有助于设计少数量的等位基因特寡核苷酸引物和双标记探针，其特地识别来自混合物的所需物种/菌株/类群中的每一个，降低化验成本。对于类群或跨种分析，当类群或类群高度不同时，该特征尤其有用。对于一组预先设计的、经过验证的引物，AlleleID可以设计兼容的引物和探针的其余序列的物种或类群特检测。
Real Time PCR
Introduction to Real Time PCR
As the name suggests, real time PCR is a technique used to monitor the progress of a PCR reaction in real time. At the same time, a relatively small amount of PCR product (DNA, cDNA or RNA) can be quantified. Real Time PCR is based on the detection of the fluorescence produced by a reporter molecule which increases, as the reaction proceeds. This occurs due to the accumulation of the PCR product with each cycle of amplification. These fluorescent reporter molecules include dyes that bind to the double-stranded DNA (i.e. SYBR® Green ) or sequence specific probes (i.e. Molecular Beacons or TaqMan® Probes). Real time PCR facilitates the monitoring of the reaction as it progresses. One can start with minimal amounts of nucleic acid and quantify the end product accurately. Moreover, there is no need for the post PCR processing which saves the resources and the time. These advantages of the fluorescence based real time PCR technique have completely revolutionized the approach to PCR-based quantification of DNA and RNA. Real time PCR assays are now easy to perform, have high sensitivity, more specificity, and provide scope for automation. Real time PCR is also referred to as real time RT PCR which has the additional cycle of reverse transcription that leads to formation of a DNA molecule from a RNA molecule. This is done because RNA is less stable as compared to DNA
Species Identification Assays/Cross Species Assays/Allele Identification Assays
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