使用期限*
许可形式单机
原产地美国
介质下载
适用平台windows
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AlleleID 物种识别和分类鉴别的综合软件
的实验设计
AlleleID是一款旨在解决、病原体检测或物种的综合性桌面工具。以CulsSTW多序列比对为核心,AlleleID可以设计用于物种识别/交叉物种的基因芯片探针以及用于实时PCR的SYBR Green, TaqMan MGB, TaqMan探针, Molecular Beacons以及实时PCR引物。AlleleID还提供了支持可变剪切片段检测的微阵列实验设计。
物种分析/跨物种实验/等位基因分析
AlleleID使用ClustalW对序列进行排列,并分析保存和物种特定区域。您可以使用程序进行实时PCR引物设计(包含SYBR Green引物设计)和双标签探针设计(TaqMan probes,
TaqMan MGB and molecular beacons)。这些检测方法只用于进行菌株检测或从混合菌株中找到感兴趣的物种。为了进行交叉物种试验,AlleleID通过识别保守区域来设计通用探针。对于相关的生物体,当研究物种的基因组草图不可用时,可以使用AlleleID来研究其基因表达。这些强大的功能可以帮助解决了很多具有挑战性的难题,例如的检测、、定量以及污染物、环境等的监测。
Sophisticated Algorithms for Assay Success
Highly specific oligos are designed by avoiding regions of significant homologies found by automatically interpreting BLAST search results. Real time PCR primer & probe efficiency is enhanced by avoiding template secondary structures. "Minimal Set", one of the most innovative features in the program, helps design the fewest number of allele specific oligonucleotide primers and dual labeled probes that uniquely identify each of the desired species/strain/taxa from the mix, lowering assay costs. For taxa or cross species assays, this feature is especially useful when the group or taxa is highly dissimilar. For a partial set of pre-designed, proven set of primers, AlleleID® can design compatible primers and probes for the rest of sequences for species identification or taxa specific assays.
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Minimal Probes with Optimal Primers
For Optimal, the program minimizes the number of primers required to amplify all sequences. The goal is to minimize the synthesis and overall assay costs.
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多路连接依赖探针扩增(MLPA)检测拷贝数和突变检测
AlleleID是设计MLPA合成探针或者由MRC Holland引入的多重连接依赖探针扩增技术的程序,当合适的工具包不可用时,研究人员能够扩展这项技术。这是一种快速增长的高通量基因图谱分析方法。可以检测外显子缺失、拷贝数变化和CpG甲基化模式。具有成本效益合适、敏感性高、特好和可再生性好等特点。
AlleleID为PamGene检测设计了mRNA特,DNA特和突变特MLPA探针。这将使PamChip用户在使用PamGene高精度检测平台时设计自己感兴趣的基因探针检测。结合这两种技术,拷贝数的变化和突变可以很*地在6小时内检测到。
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Modern Techniques for Cross Species Detection
Real time PCR and microarrays offer a rapid, sensitive, specific and quantitative tool for the diagnosis of pathogens. Diseases are not readily distinguished based on symptoms when there is more than one pathogen in the sample. Microscopic identification requires culturing on specialized media for days to weeks. Recently, real time PCR assays were successfully used in the detection of fungal pathogens such as Rhizoctonia, Pythium, and Fusarium.
Designing a cross species assay involves the use of a multiple sequence alignment tool to identify conserved stretches of DNA and the design of a hybridization probe for detecting it. Recently there have been advances in micro-organism identification at the taxonomical level using molecular level identification techniques such as real time PCR and microarrays.
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