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Evaluate Pre-Designed Primers and Probes
PrimerPlex can use pre-designed well proven oligos to build a multiplex set. After specifying the oligos for each sequence, their properties are analyzed and the user is alerted for any deviations so found. For the sequences where pre-designed oligos are not available, PrimerPlex designs them, checks them for multiplexing and highlights compatibility issues if any. The user can then decide to accept the design or create a separate pool. This functionality gives complete control in the hands of the user.
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功能
多重SNP检测和高通量基因型
使用PrimerPlex,您可以设计SNP在旁侧的多重PCR引物、SNP在3’末端的ASPE引物以及SNPs在中心的捕获探针。PrimerPlex使您能够通过它们中每一个设计oligo,来检测一个中等大小的基因组序列的所有突变。检查这些引物和探针以进行复用,并显示佳可能的。
下一代测序分析
使用PrimePlex设计多重oligos,用于预先测序的样品制备和突变检测,如SNPs,DIPs(/插入多态性)和MNPs(多核苷酸多态性),就像点击几个按钮一样简单。
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Primer Design for Standard PCR Assays
Primer Premier is the most comprehensive software to design and analyze PCR primers.
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"Premier Biosoft can make good software. For me Primer Premier is the best oligo design program." -Dr. Rimantas Kodzius, Genome Science Laboratory, The Institute of Physical and Chemical Research (RIKEN) Itama, Japan.
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