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Data & Database Management
Multiple projects can be created. Data of multiple experiments can be easily managed by creating separate project for each experiment. Maintains a local database for sequence information and search results.
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Edits Template sequences to add new
bases or delete unwanted regions.
Exports a publication quality report for
the assays designed for sharing with
colleagues.
Includes a built-in calculator for
computing the GC%, the base count
and to obtain the complement and the
reverse complement.
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Primer Premier PCR引物设计软件
自动同源性和避免模板结构
Primer Premier自动解释BLAST搜索结果,并避免那些区域设计与数据库具有显著交叉同源性的引物。这些同源区域在序列视图中被**显示,并且在引物搜索期间被避免了。由于在扩展温度下存在模板结构,可能会阻碍引物的扩展。为了避免所有这些区域的模板自动折叠,在您的折叠温度下,程序使用专有算法来检查模板内可能的二级结构。在序列视图中,二级结构形成的区域下面用横线强调,并在设计引物时被避免。避免同源区域使得寡聚物高度特,避免模板结构提高设计引物的效率。
手动引物搜索
Primer Premier为用户提供对引物设计的完全控制。使用手动搜索选项,用户可以选择模板序列上的任何位置来设计感测或反义引物。Primer Premier会立即计算并显示新引物的性能。然后在主窗口的序列视图中显示由用户终选择的引物。
A Comprehensive PCR Primer Design Software
Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in a ranked order. Load the gene of interest from NCBI, select a search range, sit back and let Primer Premier pick the best possible primers for you.
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Sequence View
The selected sequence can be viewed in 3 or 10 bases per block, single stranded or double stranded. The designed primers are marked, the sense primer in blue and the antisense primer in red. The selected SNPs are marked in pink. The homologies regions are highlighted in yellow and the bases participating in template structures are underlined. You can export the "sequence view" along with the primer design results in csv or HTML format.
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