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"Yes, we are currently using Primer Premier and are getting excellent, i.e., consistent and repeatable, results using the primers designed with your software" -Dr. Dave Alexander, Nagoya City University, Japan.
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"I became interested in Beacon Designer™ because of some articles I had read in trade literature. Once I ran through the tutorial-kudos to the team who designed it--one of the best I've seen for software of this type. I then was able to design 8 sets of primers using the software. I found the software user friendly, and if I got stuck I replayed the applicable tutorial section. I contacted customer support, and the service far exceeded my expectations. The reason we chose PREMIER Biosoft was because it was highly recommended to us as a highly service oriented company and now after talking to the support, I've seen why that was said." -Mr. Richard Hanes, Molecular Biology Core Lab Manager, Bowles Center, UNC-Chapel Hill Medical School.
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PrimerPlex 多重PCR和高通量SNP基因分型和分析的Oligo设计
对于SNP的基因型研究,PrimerPlex设计了SNP的旁侧引物。其结果包括每个DNA模板的引物对,包括产品尺寸、退火温度等物理化学寡聚物性能。此外,二级结构的图形显示也可用以帮助确定它们是否会阻碍反应。
多重PCR检测用于基因表达分析(使用凝胶电泳的终点检测),用于高通量SNP应用,如基因分型,用于下一代测序所需的目标浓缩、病原体检测、菌株分型和单倍型分型。PrimerPlex还设计了优等位基因特捕捉探针和Allele Specific Primer Extension (ASPE)引物用于高通量SNP基因型分析。
Primer Specificity
Evaluate Pre-Designed Primers and Probes
Addition of MagPlex TAGs
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PrimerPlex是一种、复杂的工具,用于设计oligo并进行多重分析。多重分析促进了单个反应容器中多个目标的扩增,减少了实验的时间和成本。
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