使用期限*
许可形式单机
原产地美国
介质下载
适用平台windows
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Real Time PCR VS Traditional PCR
Real time PCR allows for the detection of PCR product during the early phases of the reaction. This ability of measuring the reaction kinetics in the early phases of PCR provide a distinct advantage over traditional PCR detection. Traditional methods use gel electrophoresis for the detection of PCR amplification in the final phase or at end-point of the PCR reaction.
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Modern Techniques for Cross Species Detection
Real time PCR and microarrays offer a rapid, sensitive, specific and quantitative tool for the diagnosis of pathogens. Diseases are not readily distinguished based on symptoms when there is more than one pathogen in the sample. Microscopic identification requires culturing on specialized media for days to weeks. Recently, real time PCR assays were successfully used in the detection of fungal pathogens such as Rhizoctonia, Pythium, and Fusarium.
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Specific Detection using Target Specific Probes
Specific detection of real time PCR is done with some oligonucleotide probes labeled with both a reporter fluorescent dye and a quencher dye. Probes based on different chemistries are available for real time detection, these include:
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AlleleID 物种识别和分类鉴别的综合软件
的实验设计
AlleleID是一款旨在解决、病原体检测或物种的综合性桌面工具。以CulsSTW多序列比对为核心,AlleleID可以设计用于物种识别/交叉物种的基因芯片探针以及用于实时PCR的SYBR Green, TaqMan MGB, TaqMan探针, Molecular Beacons以及实时PCR引物。AlleleID还提供了支持可变剪切片段检测的微阵列实验设计。
物种分析/跨物种实验/等位基因分析
AlleleID使用ClustalW对序列进行排列,并分析保存和物种特定区域。您可以使用程序进行实时PCR引物设计(包含SYBR Green引物设计)和双标签探针设计(TaqMan probes,
TaqMan MGB and molecular beacons)。这些检测方法只用于进行菌株检测或从混合菌株中找到感兴趣的物种。为了进行交叉物种试验,AlleleID通过识别保守区域来设计通用探针。对于相关的生物体,当研究物种的基因组草图不可用时,可以使用AlleleID来研究其基因表达。这些强大的功能可以帮助解决了很多具有挑战性的难题,例如的检测、、定量以及污染物、环境等的监测。
广泛支持实时或定量PCR检测和SNP/表达微阵列
AlleleID可以设计优的SYBR Green引物、TaqMan探针、TaqMan Minor Groove Bingding
(MGB)探针、FRET探针或者分子信标,用于实时定量PCR差异基因表达SNP基因型分类试验。您也可以设计实时PCR引物和双标记探针多达一万个序列在一个单一的运行SNP检测或表达微阵列。
AlleleID®
Design qPCR and Microarray Assays for Related Organisms
AlleleID® is a comprehensive desktop tool designed to address the challenges of bacterial identification, pathogen detection or species identification. With ClustalW multiple sequence alignment at its core, AlleleID® can be used to design species identification/cross species probes for microarrays or real time PCR including SYBR® Green, TaqMan® MGB, TaqMan® probes, Molecular Beacons and real time PCR primers. AlleleID® also offers support for designing microarray experiments for detecting alternative splicing events.
Cross Species/Taxa Specific Assays
Species Identification Assays
Gene Splicing/Alternative Splicing using Microarrays
Exon Junction Primer Design
NEAT Assay Design
Database Support
Input & Output Formats
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