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多重PCR检测用于基因表达分析（使用凝胶电泳的终点检测），用于高通量SNP应用，如基因分型，用于下一代测序所需的目标浓缩、病原体检测、菌株分型和单倍型分型。PrimerPlex还设计了优等位基因特捕捉探针和Allele Specific Primer Extension (ASPE)引物用于高通量SNP基因型分析。
"Beacon Designer is very easy to use and worked really well for the designing of reverse-transcription quantitative PCR protocol. I am satisfied with the product and it addressed my research needs well." -Ms. Eleonora Sittig, Turku Center for Biotechnology, Finland.
Avoiding homologous regions makes the oligos highly specific and avoiding template structures improves the efficiency of the designed primers.
Evaluate Pre-Designed Primers and Probes
PrimerPlex can use pre-designed well proven oligos to build a multiplex set. After specifying the oligos for each sequence, their properties are analyzed and the user is alerted for any deviations so found. For the sequences where pre-designed oligos are not available, PrimerPlex designs them, checks them for multiplexing and highlights compatibility issues if any. The user can then decide to accept the design or create a separate pool. This functionality gives complete control in the hands of the user.
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