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Limitations of End-point PCR
In a PCR reaction as the reaction progresses, the reagents are being consumed as a result of amplification. Now the PCR product is no longer being doubled at each cycle due to this reagent constraint. This depletion will occur at different rates for each replicate. Thus, the samples begin to diverge in their quantities. This diminished amplification is the linear phase of the reaction. The plateau for each tube will differ due to the different reaction kinetics for each sample. It is in this phase where traditional PCR takes its measurement, also known as the end-point. This End-Point Detection has some problems such as low resolution, poor precision, low sensitivity and the need for post PCR processing. Also, the results of this detection are not expressed in numbers and there is no scope for automation.
Species Identification
Using AlleleID®, you can design assays for identifying species and strains from a mix of sequences
Molecular based techniques use species-specific probes, designed for specific regions of the target gene, for the detection and identification of bacteria. Microarrays can rapidly detect and identify multiple pathogens simultaneously in complex backgrounds.
Species Identification Assays/Cross Species Assays/Allele Identification Assays
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