使用期限*
许可形式单机
原产地美国
介质下载
适用平台windows
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Beacon Designer™ is a comprehensive real time PCR primer and probe design tool for designing single template and multiplex assays. Real time PCR chemistries supported include Molecular beacons, TaqMan®, FRET, Scorpions® and SYBR® Green. It designs molecular beacons for standard and NASBA® assays and designs LNA spiked Taqman® probes as well. AlleleID® supports all the major features of Beacon Designer™ except NASBA® assay, LNA™ spiked Taqman® probe design and multiplex assay design. TaqMan® MGB probe design is unique to AlleleID® (whereas regular TaqMan® probes based assays can be designed using both AlleleID® and Beacon Designer™. AlleleID® is written for advance real time PCR applications such as oligo design for species identification and taxa discrimination. It includes a multiple sequence alignment module for identifying conserved and species specific regions. AlleleID® also includes support for real time PCR primer design over exon junctions for selective amplification of cDNA.
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Real Time PCR Applications Include
1 . Quantitative mRNA expression studies.
2 . DNA copy number measurements in genomic or viral DNAs.
3 . Allelic discrimination assays or SNP genotyping.
4 . Verification of microarray results.
5 . Drug therapy efficacy.
6 . DNA damage measurement.
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Limitations of End-point PCR
In a PCR reaction as the reaction progresses, the reagents are being consumed as a result of amplification. Now the PCR product is no longer being doubled at each cycle due to this reagent constraint. This depletion will occur at different rates for each replicate. Thus, the samples begin to diverge in their quantities. This diminished amplification is the linear phase of the reaction. The plateau for each tube will differ due to the different reaction kinetics for each sample. It is in this phase where traditional PCR takes its measurement, also known as the end-point. This End-Point Detection has some problems such as low resolution, poor precision, low sensitivity and the need for post PCR processing. Also, the results of this detection are not expressed in numbers and there is no scope for automation.
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Design qPCR and Microarray Assays for Related Organisms
AlleleID® is a comprehensive desktop tool designed to address the challenges of bacterial identification, pathogen detection or species identification. With ClustalW multiple sequence alignment at its core, AlleleID® can be used to design species identification/cross species probes for microarrays or real time PCR including SYBR® Green, TaqMan® MGB, TaqMan® probes, Molecular Beacons and real time PCR primers. AlleleID® also offers support for designing microarray experiments for detecting alternative splicing events.
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