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How Species Were Identified Historically
The field of disease diagnosis and drug development has seen a rapid replacement of culture based methods for bacterial and species identification by faster and more reliable molecular based techniques such as real time PCR and microarrays. The reason for the success for these techniques is the ease with which one can identify a species from a sample collected from complex sources such as air, soil, or water.
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Modern Techniques for Cross Species Detection
Real time PCR and microarrays offer a rapid, sensitive, specific and quantitative tool for the diagnosis of pathogens. Diseases are not readily distinguished based on symptoms when there is more than one pathogen in the sample. Microscopic identification requires culturing on specialized media for days to weeks. Recently, real time PCR assays were successfully used in the detection of fungal pathogens such as Rhizoctonia, Pythium, and Fusarium.
Designing a cross species assay involves the use of a multiple sequence alignment tool to identify conserved stretches of DNA and the design of a hybridization probe for detecting it. Recently there have been advances in micro-organism identification at the taxonomical level using molecular level identification techniques such as real time PCR and microarrays.
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AlleleID 物种识别和分类鉴别的综合软件
的实验设计
AlleleID是一款旨在解决、病原体检测或物种的综合性桌面工具。以CulsSTW多序列比对为核心,AlleleID可以设计用于物种识别/交叉物种的基因芯片探针以及用于实时PCR的SYBR Green, TaqMan MGB, TaqMan探针, Molecular Beacons以及实时PCR引物。AlleleID还提供了支持可变剪切片段检测的微阵列实验设计。
功能特征
物种试验
基因芯片的基因拼接/选择性剪接
外显子连接引物设计
实时PCR引物设计
TaqMan探针设计
SyBR绿色底漆设计
分子信标设计
FRET探针设计
微阵列探针设计
精密化验设计
数据库支持
输入输出格式
ross Species/Taxa Specific Assays
Species Identification Assays
Gene Splicing/Alternative Splicing using Microarrays
Exon Junction Primer Design
Real Time PCR Primer Design
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Choices for Primer Design: There are two choices available for primer design, Unique and Optimal. When you choose Unique, the program designs a primer pair to amplify each sequence. For Optimal, the program minimizes the number of primers required to amplify all sequences. The goal is to minimize the synthesis and overall assay costs.
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