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Sequence handling: Import of sequences from a number of different formats with direct database search in Entrez and in local databases (FastA format and Swiss-Prot). Sequences can be saved in local files (databases) for future reference.
From the sequence window a large number of actions can be performed. Sequences can be exported in FastA format (either singly or all sequences at once) for easy transfer to other programs.
Mass analysis: The protein can be cleaved by automatic methods (e.g. a flexible nomenclature for defining enzyme actions) or manually. The peptides are displayed with a number of parameters (various mass values - mono, ave, charges - Bull&Breese index, HPLC index, pI, charge) and can be further worked upon (e.g. cross-linked, new cleavage).
Peptide mass searches can be performed on any local database in FastA format.
Bioinformatics: A number of graphs can be displayed, hydrophobicity, dot-plot, secondary structure prediction. BLAST searches can be performed on local databases. Multiple alignment using ClustalW.
The CustalW executable is now part of the GPMAw package, but on previous versions you have to install yourself. Read here how.
![GPMAW正版软件如何用](//l.b2b168.com/2020/06/18/15/202006181534436222934.jpg)
程序内容
GPMAW的文档在帮助栏中提供,手册可以在下载。
位置
液相色谱指数
理论PI
布尔和布雷斯指数
序列-1/3字母等
![GPMAW正版软件如何用](//l.b2b168.com/2019/07/18/10/201907181030385050684.jpg)
The scissors are a shortcut to cutting up the protein into peptides. This is usually done using proteolytic enzymes, but may also be carried out chemically. The only requirement is that the process can be specified relative to specific residues. The small down-arrow button opens a menu enabling you to select enzyme cleavage in a single click. Note that the bottom part of the menu enables you to specify one missed cleavage, and/or to digest all opened sequence windows.
![GPMAW正版软件如何用](//l.b2b168.com/2019/07/18/10/201907181030352586494.jpg)
序列处理:通过在Entrez和本地数据库(FastA格式和Swiss-Prot)中直接搜索数据库,从许多不同格式的序列中导入序列。序列可以保存在本地(数据库)中,以备将来参考。从序列窗口可以执行大量操作。序列可以以FastA格式导出(单个序列或同时导出所有序列),以便于传输到其他程序。
质量分析:蛋白质可以通过自动方法(如定义酶作用的灵活命名法)或手动方法进行裂解。多肽显示与许多参数(各种质量值- mono, ave,电荷- Bull&Breese指数,HPLC指数,pI,电荷),并可以进一步工作(如交联,新的分裂)。
生物信息学:可显示多种图形、疏水性、点图、二级结构预测。可以在本地数据库上执行BLAST搜索。使用ClustalW的多重对齐。CustalW可执行文件现在是GPMAw包的一部分,但是在以前的版本中,您必须自己安装。
序列窗口
序列窗口是GPMAW的核心窗口并默认显示一个序列。从这里,您可以调用大多数与序列相关的函数(通过菜单、工具栏或弹出菜单)。
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