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适用平台Windows
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A5 The leftmost panel shows the total mass of the protein in Daltons. The button next to it shows whether it is the average mass (Av. – blue) or monoisotopic mass (Mo. – red). You can change the mass type by clicking on the Av./Mo. button.
The next two panels from the left can show either of two states:
A6 If no peptide is selected the mass of the amino acid under the cursor (i.e. the residue mass + 18 Da) will be displayed in the left-hand panel and the right-hand panel will show the residue number (if the protein is a multi-chain protein, the first chain will be labeled ‘a’, the second ‘b’ etc (i.e. 80b is residue number 80 in the second chain counting from the N-terminus).
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质量分析:蛋白质可以通过自动方法(如定义酶作用的灵活命名法)或手动方法进行裂解。多肽显示与许多参数(各种质量值- mono, ave,电荷- Bull&Breese指数,HPLC指数,pI,电荷),并可以进一步工作(如交联,新的分裂)。
生物信息学:可显示多种图形、疏水性、点图、二级结构预测。可以在本地数据库上执行BLAST搜索。使用ClustalW的多重对齐。CustalW可执行文件现在是GPMAw包的一部分,但是在以前的版本中,您必须自己安装。
The GPMAW program is primarily intended as a tool for mass spectrometric analysis of proteins and peptides. However, a number of other bioinformatics tools have been included, so the use of the program extends far beyond simple mass analysis.
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‘Invert sequence’ will result in highlighting of sequences found in both N- and C-terminal directions (e.g. if entering KLGFT both the sequence KLGFT and the sequence TFGLK will be highlighted). Useful for searching for a ms/ms sequence tags (you may not know whether it is a y-ion or a b-ion series). Isobaric resides will, if checked, highlight both kinds of residues, i.e. if the ‘Q/K’ checkbox is checked and the sequence LKT is entered, the sequence LQT will also be highlighted. Again, this is for the benefit of ms/ms sequence tags.
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显示1个或3个字母
平均质量/单同位素质量
固定残留物宽度
带有序列信息的提要栏
显示二硫化物桥、改性残留物等
通过**显示部分序列,您可以轻松获得给定肽的质量。为了便于导航,您可以使用特定颜色的残留物(提供三种不同颜色和下划线)。大多数设置可以很*地在系统设置框中预先设置。
序列窗口形成父窗口,从中可以创建大量派生子窗口:
肽窗口
MS/MS窗口
批量搜索、合成搜索
图表:疏水性、二级结构、电荷与酸碱度、点图、α螺旋轮
肽窗口
肽窗口通常通过自动摘要命令从序列窗口中调用。但是,也有其他方法,如手动或半自动分裂。
Sequence window
The sequence window is the central window of GPMAW and default view of a sequence. From here you can call most of other sequence related functions (either through the menu, the toolbar, or the pop-up menu).
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