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具体而有效的设计:Beacon Designer如何使其成为可能
您可以在程序中使用BLAST搜索序列并搜索模板结构。在设计引物和探针时使用这两种搜索的结果。在设计过程中,会自动避免出现明显的交叉同源性和模板结构的区域。
功能特征
SYBR Green引物设计
TaqMan探针设计
多路实时PCR检验设计
HRMA引物设计
MethyLight TaqMan设计
分子信标的设计
NASBA检验设计
FRET探针设计
Scorpions设计
避免交叉同源性-针对特定的Oligo设计
避免模板结构——提高引物效率
Oligo评级
![Beacon designer软件安装教程](//l.b2b168.com/2021/09/27/17/202109271731574380764.jpg)
Beacon Designer™ outputs many TaqMan® probe-primer sets for the user to choose from.
![Beacon designer软件安装教程](//l.b2b168.com/2021/09/27/17/202109271731574333014.jpg)
You can use pre-designed or published or well proven forward or reverse primers and Beacon Designer™ will evaluate them for you. For example, for a given forward primer, Beacon Designer™ would design the reverse primer for SYBR® Green assays (and would design both the reverse primer and the TaqMan® probe for TaqMan® assays). All these primers are rated according to a ranking algorithm which determines how closely the specified primer pair and primer search parameters match the target values.
![Beacon designer软件安装教程](//l.b2b168.com/2021/09/27/17/202109271731574091354.jpg)
多路实时PCR检验
有了Beacon Designer,您可以设计基于TaqMan的多路复用实时PCR检验,包括参考基因选择。
功能特征
SYBR Green引物设计
TaqMan探针设计
多路实时PCR检验设计
HRMA引物设计
MethyLight TaqMan设计
分子信标的设计
NASBA检验设计
FRET探针设计
Scorpions设计
避免交叉同源性-针对特定的Oligo设计
避免模板结构——提高引物效率
Oligo评级
Beacon Designer™
Design specific and efficient oligos for all major qPCR assays
Design successful SYBR® Green, TaqMan®, HRMA Primers, MethyLight TaqMan®, LNA™ spiked TaqMan®, molecular beacons for both standard and NASBA® assays, Scorpions® and FRET assays by automatically interpreting BLAST results and avoiding template structures.
Automate Your Real Time PCR Primer and Probe Design
Beacon Designer™ automates the design of real time primers and probes. It is used by molecular biologists worldwide to design successful real time PCR assays. It saves the time and the money involved in failed experiments. Beacon Designer™ is a flexible solution to your real time primer and probe design needs and pays for itself many times over.
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