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"I am a long time user of the Primer Premier 5, and I always used it with great success" -Stefan Hauptfleisch.
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Molecular dysexpression in gastric cancer revealed by integrated analysis of transcriptome data
Xiaomei Li, Weiwei Dong, Xueling Qu, Huixia Zhao, Shuo Wang, Yixin Hao, Qiuwen Li, Jianhua Zhu, Min Ye, Wenhua Xiao.
Oncology Letters. 2017; May;13(5):3177-3185. doi: 10.3892/ol.2017.5798.
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"Beacon Designer is very easy to use and always gives good primers." -Dr. Chenggang Wu, The University of Texas Health Science Center at Houston, US.
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Automatic Homology & Template Structure Avoidance
Primer Premier automatically interprets the BLAST search results and avoids those regions to design primers that have significant cross homologies with the database. These homologous regions are highlighted in the sequence view and are avoided during primer search.
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