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"We have designed several primer/probe sets for reverse-transcription quantitative PCR using TaqMan. All have worked very well. We particularly like the way that the primers/probes are 'scored' as best, good or poor, it is much more convenient than other programs we have used. We also like the integrated BLAST feature. We are very satisfied customers and have recommended the product to our colleagues and collaborators." -Ms. Laura O'Hara, University of Cambridge, UK.
"I truly enjoyed the program over all these years (see ref. in Fetissov et all., Reg. Peptides 119:113-124, 2004)." -Dr. Serguei Fetissov, Karolinska Institute, Sweden.
引物的延伸可能会由于在延伸温度下模板结构的存在而受阻。为了避免所有这些区域，模板可能折叠在自己，该程序利用一个专有的算法来检查模板中可能的二级结构在您*的折叠温度。
"AlleleID is extremely easy to use. I hardly needed to go through the tutorial and the Help section was so comprehensive that it answered all my questions." -Dr. Chantal Niekerk, University of Pretoria, South Africa.
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